ISSN: 0034-8376
eISSN: 2564-8896






Serum Repressor Element-1 Silencing Transcription Factor Levels in Alzheimer?s Patients from a National Institute of Health in Mexico City, Elderly and Young Controls



Francisco L. Ramírez-Cuapio, Dementia Laboratory, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez (INNNMVS), Mexico City; Master's Program in Chemical-Biological Sciences, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City; Mexico
Mónica A. Torres-Ramos, Neuroscience Peripheral Unit, INNNMVS/Universidad Nacional Autónoma de México, Mexico City, Mexico
Marisol Orozco-Ibarra, Laboratory of Molecular and Cellular Neurobiology, INNNMVS, Mexico City, Mexico
Isaac Acosta, Dementia Laboratory, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez (INNNMVS), Mexico City, Mexico
Ana L. Sosa-Ortiz, Dementia Laboratory, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez (INNNMVS), Mexico City, Mexico


Background: Decreased levels of repressor element-1 silencing transcription (REST) factor in the brain, plasma, and neuronderived exosomes are associated with Alzheimer’s disease (AD). Objective: The objective of the study was to test the viability of serum REST as a possible blood-based biomarker for AD, comparing serum REST levels in AD patients from a National Institute of Health in Mexico City (with different levels of severity and comorbidities), with elderly controls (EC) and young controls (YC). Methods: We used an enzyme-linked immunosorbent assay to determine serum REST levels in AD patients (n = 28), EC (n = 19), and YC (n = 24); the AD patients were classified by dementia severity and comorbidities (depression and microangiopathy) using clinimetric tests and magnetic resonance imaging. Results: Mean serum REST levels did not differ between AD patients, EC, and YC. The severity of AD and the presence of depression or microangiopathy were not associated with serum REST levels. Conclusion: Our results differ from previously published patterns found for plasma and cerebral REST levels. Free serum REST levels may not be a viable AD blood-based biomarker.



Keywords: Aging. Blood-based biomarkers. Dementia. Non-invasive diagnostic tools. Neural-restrictive silencer factor. RE-1 silencing transcription factor.