ISSN: 0034-8376
eISSN: 2564-8896






Ethical Considerations in Animal Research: The Principle of 3R?s



Lorenza Díaz, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Elena Zambrano-González, Ethics Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
María E. Flores, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
Mariela Contreras, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Jose C. Crispín, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Gabriela Alemán, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
César Bravo, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Alejandra Armenta-Espinosa, Ethics Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
Victor J. Valdés, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City; Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico
Armando Tovar, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Gerardo Gamba, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
Jorge Barrios-Payán, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Norma A. Bobadilla, The Institutional Animal Care and Use Committee, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico


In the last century, progress in the knowledge of human diseases, their diagnosis and treatment have grown exponentially, due in large part to the introduction and use of laboratory animals. Along with this important progress, the need to provide training and guidance to the scientific community in all aspects related to the proper use of experimental animals has been indispensable. Animal research committees play a primary role in evaluating experimental research protocols, from their feasibility to the rational use of animals, but above all in seeking animal welfare. The Institutional Committee for the Care and Use of Animals (IACUC) has endeavored to share several relevant aspects in conducting research with laboratory animals. Here, we present and discuss the topics that we consider of utmost importance to take in the account during the design of any experimental research protocol, so we invite researchers, technicians, and undergraduate and graduate students to dive into the fascinating subject of proper animal care and use for experimentation. The main intention of these contributions is to sensitize users of laboratory animals for the proper and rational use of them in experimental research, as well as to disseminate the permitted and unpermitted procedures in laboratory animals. In the first part, the significance of experimental research, the main functions of IACUC, and the principle of the three R’s (replacement, reduction, and refinement) are addressed.



Keywords: Institutional Animal Care and Use Committee. Reduction. Refinement. Replacement.